Journal: International Journal of Molecular Medicine

Article Title: SENP1 promotes p27kip1 nuclear export though enhanced SUMOylation in cholangiocarcinoma leading to increased cell proliferation and chemoresistance

doi: 10.3892/ijmm.2025.5582

Figure Lengend Snippet: SUMOylation levels of nuclear and cytoplasmic p27kip1 in LMB-treated cells. (A) LMB treatment restricted the p27kip1 inside the nucleus of SENP1-oe RBE cells. (B) LMB-treated RBE cells showed significantly increased levels of nuclear SUMOylated p27kip1, but no significant changes in cytoplasmic SUMOylated p27kip1 expression levels. (C) LMB treatment restricted the p27kip1 inside the nucleus of siSENP1 RBE cells. (D) LMB-treated siSENP1 RBE cells showed significantly decreased levels of nuclear SUMOylated p27kip1, but no significant changes in cytoplasmic SUMOylated p27kip1 expression levels. Error bar, mean ± SEM. ** P<0.01, *** P<0.001, **** P<0.0001 and ***** P<0.00001. ns, not significant; SENP1, SUMO specific peptidase 1; VEC, empty vector lentivirus; si, small interfering; oe, overexpression; LMB, leptomycin B.

Article Snippet: For nuclear export inhibition assays, cells were treated with 20 nM LMB (Beyotime Institute of Biotechnology), to block the p27kip1 transporter CRM1, at 37°C for 12 h in 10% FBS in medium, which was then used for nuclear and cytoplasmic protein separation, WB and SUMOylation tests.

Techniques: Expressing, Plasmid Preparation, Over Expression